Composite

Part:BBa_K2637044:Design

Designed by: Lei Yu   Group: iGEM18_Tianjin   (2018-10-05)


Limonene sythnase cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 581


Design Notes

Limonene is a volatile monoterpenoid compound and produces a lemon-like odor with the effect of repelling mosquitoes. We builted cell factory working with the KaiABC circadian clock system has achieved to periodically produce limonene during subjective nights only to help repel mosquitoes.

Source

The Gal1 promoter is a mutant of the origin one in Saccharomyces cerevisiae. The limonene sythase gene is from the kit. ADH1 terminator is from Saccharomyces cerevisiae.

References